Revisiting in vivo staining with alizarin red S - a valuable approach to analyse zebrafish skeletal mineralization during development and regeneration
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منابع مشابه
Modified double skeletal staining protocols with Alizarin red and Alcian blue in laboratory animals
Abstract Purpose: Skeletal staining is a way to study the effect of many chemical or herbal substances on development of bones and cartilages in order to record the level of probable deformity.This article aimed to present a modified protocol to make all skeletal studies on laboratory animals possible. Materials and Methods: The important notes about skeletal staining were fixing by ethan...
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Purpose: This study designed to introduce Alizarin red S staining instead of Hematoxylin and Eosin staining in diagnosis of calcification of the paraffin sections. Materials and Methods: Uterine paraffin sections belong to the 20 women aged 53±4, endured hysterectomy stained with H&E and ARS staining. For H&E, the sections were treated with Mayer's hematoxylin (pH 2.3) for 5 min and with Eosin...
متن کاملSkeletal examination by alizarin staining.
A skeletal examination of fetuses is required in regulatory embryo-fetal development studies. This chapter describes a method of skeletal examination using alizarin staining. All fetuses are removed from the mother by caesarean section before birth. The fetuses are first examined externally. For larger species (rabbit and minipig), an internal examination can be performed on the fresh soft tiss...
متن کاملmodified double skeletal staining protocols with alizarin red and alcian blue in laboratory animals
abstract purpose: skeletal staining is a way to study the effect of many chemical or herbal substances on development of bones and cartilages in order to record the level of probable deformity.this article aimed to present a modified protocol to make all skeletal studies on laboratory animals possible. materials and methods: the important notes about skeletal staining were fixing by ethanol and...
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the frame semantic theory is a nascent approach in the area of translation studies which goes beyond the linguistic barriers and helps us to incorporate cognitive and cultural factors to the study of translation. based on rojos analytical model (2002b), which centered in the frames or knowledge structures activated in the text, the present research explores the various translation problems that...
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ژورنال
عنوان ژورنال: BMC Developmental Biology
سال: 2016
ISSN: 1471-213X
DOI: 10.1186/s12861-016-0102-4